Figure 2.

A, Evaluation of amastigote transformation dynamics of parasite morphology and cell viability in samples stained with trypan blue. B, reverse transcription polymerase chain reaction analysis of expression of A2 amastigote-specific protein in axenic Leishmania infantum chagasi amastigotes. a, A2 primer; b, α-tubulin primer. Lanes 1–4 = axenic amastigotes 24, 48, 72, and 96 hours post in vitro incubation, respectively; lane 5 = cultivated promastigotes; lane 6 = negative control. The dataset represent the analysis of two independent experiments. bp = basepairs. Error bars indicate mean ± SD.