Table 7.
Corrected† IL-8 levels in serum (pg/mL) N° responding pigs; Average ± standard deviation |
|||||
---|---|---|---|---|---|
Experiment 1 Group |
Days post-challenge |
||||
|
1* |
2* |
3 |
7¶ |
14 |
C1 + B |
2/2a 270 ± 108 |
2/2a 424 ± 255 |
2/2a 440 ± 434 |
2/2a 470 ± 606 |
1/2 711 ± N.A. |
A + A |
0/4 N.A. |
1/4 440 ± N.A. |
2/4 272 ± 363 |
1/4 822 ± N.A. |
1/4 124 ± N.A. |
A + B |
2/4 644 ± 479 |
3/4 194 ± 98 |
0/4 N.A. |
0/4 N.A. |
0/4 N.A. |
Experiment 2 Group |
Days post-challenge |
||||
|
1 |
2 |
3 |
7 |
14¶ |
C2 + A |
0/3b N.A. |
0/3b N.A. |
0/3b N.A. |
0/3b N.A. |
0/3 N.A. |
B + B |
3/4 249 ± 192 |
1/4 526 ± N.A. |
2/4 212 ± 211 |
2/4 262 ± 247 |
3/4 297 ± 273 |
B + A | 2/3 38 ± 2 |
1/3 667 ± N.A. |
2/3 1088 ± 407 |
1/3 577 ± N.A |
3/3 291 ± 117 |
† In order to account the pre-2nd challenge levels of a given cytokine, values (pg/mL) obtained with samples of day 0 (84 pi) (C1 + B:653 ± 66; A + A:796 ± 306; A + B:1062 ± 157; C2 + A:503 ± 133; B + B:187 ± 198; B + A:41.3 ± 0) were subtracted to the values obtained at days 1, 2, 3, 7 or 14, respectively for producing a corrected value (Corrected value for cytokine X = Concentration of cytokine X day n - Concentration of cytokine X day 0).
N.A. not applicable. Symbols (* p = 0.05; ¶p < 0.05) show days for which statistical differences were observed between groups of a given experiment with regards to the proportion of positive pigs. Letters indicate statistically significant differences for the proportion of responding pigs for similar groups when comparing between experiments; i.e. naïve pigs inoculated with 3267 (C1 + B) vs. naïve pigs inoculated with 3262 (C2 + A). In this case, a,b correspond to p ≤ 0.1.