Figure 1.

Overview of the prediction framework. Diagram representing the workflow of bioinformatic prediction of putative OMPs from the avian and porcine strain genomes of P. multocida. Ten predictors were categorized into 3 groups: subcellular localization, transmembrane β-barrel protein prediction and outer membrane lipoprotein prediction. The predicted proteins in each group were filtered by consensus prediction and combined to form a single integrated list. Text mining and sequence analyses were used to confirm that the predicted proteins were outer membrane-associated with a high degree of confidence. The proteins filtered-out from the avian strain genome were also analyzed further by data mining and sequence analysis. The numbers of predicted proteins in each step are shown in parentheses: the first number represents proteins from the avian strain genome and the second number from the porcine strain genome.