Table 1.

Sense (F) and antisense (R) oligonucleotides used for PCR amplifications

Primer	Sequence (5’ – 3’)	Hybridization site
AscI_GI_F	TAGGCGCGCCATGGTCCGTCCTGTAGAA	5’ terminus of GUS-intron
GI_NotI_R	TTGCGGCCGCAGAGGATCCTCATTGTTT	3’ terminus of GUS-intron
Rep_F	GCCTGAGTTTTGTGGTTGG	internal to PVX RdRp
GI_R	GGATAGTCTGCCAGTTCAGTTCG	internal to GUS-intron
GI_XhoI_R	CCTACTCGAGCCTCATTGTTTGCCTCCC	3’ terminus of GUS-intron
PEBVsgPR_F	AACTCGGTTTGCTGACCTAC	PEBV subgenomic promoter
TRV3UTR_R	ACCTAAAACTTCAGACACGG	3’ UTR of TRV RNA-2
AgeI-GI_F	TATAACCGGTCATGGTCCGTCCTGTAGAAACC	5’ terminus of GUS-intron
pEAQ_F	AACGTTGTCAGATCGTGCTTCGGCACC	5’ UTR of pEAQ-HT
pEAQ_R	CTCCTGTTTAGCAGGTCGTCCCTTCAG	3’ UTR of pEAQ-HT

Sequences added with 5’ extensions are shown in bold with restriction enzyme recognition sites underlined. Initiation codons are italicized. UTR, untranslated region; PEBV, Pea early browning virus; RdRp, RNA-dependent RNA polymerase.