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. 2012 May 25;12:25. doi: 10.1186/1472-6750-12-25

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Copyright ©2012 Bonde and Bulow; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SDS-PAGE analysis of encapsulation experiments with amelogenin (AMG) mixed with BSA (Panel A) or insulin (Ins.) (Panel B). By changing the pH from weakly acidic to neutral the aggregation of amelogenin is induced, which entraps the target molecule. Proteins captured in the aggregates are released upon acidification will end up in the solubilized pellet sample (P). The soluble fraction (S) contains proteins not precipitated or captured by the neutralization. The three subpanels are taken from the same gel. Blue arrowsindicate amelogenin, green arrow indicates BSA, and red arrow indicates insulin.