Table 3.
Primers used for RT-PCR and nucleotide sequencing.
| primer | location1 |
used for |
sequence (5´- > 3´) | |
|---|---|---|---|---|
| PCR | sequencing | |||
| P100 |
99-121 |
PCR1 |
+ |
CATGCCCTTAGTAGGACTAGCAC |
| CSF1400r |
1401-1419 |
PCR1 |
+ |
CACCAYCCRTGTTTRTTCC |
| CSF1300f |
1280-1299 |
PCR2 |
+ |
AAAATATGYAARGGRGTCC |
| CSF2400r |
2396-2415 |
PCR2 |
+ |
AGCCATAYYAYACCTTGCAC |
| CSF2250f |
2224-2243 |
PCR3 |
+ |
TGTTAGRCCRGRYTGGTGGC |
| CSF3710r |
3708-3728 |
PCR3 |
+ |
TRGTYTTRACTGGRTTGTTRG |
| CSF3000r |
2988-3009 |
- |
+ |
TTYACACATGTCCARTTRCCCC |
| CSF3010f |
2990-3012 |
- |
+ |
GGYAAYTGGACATGTGTRAAAGG |
| CSF2919f | 2900-2919 | - | + | ACCTTCAGGAGAGATAAGCC |
1 Numbers refer to the sequence of CSFV strain Alfort187 [GenBank: X87939].