Figure 7.

Hydrolytic-activity and western-blot analyses of PM H⁺-ATPase from cucumber roots (A) or leaves (B). The plants were treated as described in the legend of Figure 4. Data are means ± SD of three independent experiments with three replicates. Immunodetection of the enzyme isolated from the three independent experiments was performed using antibodies raised against the C-terminal part of the Arabidopsis AHA3 PM H⁺-ATPase. Blots of a representative experiment are shown.