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. 2012 May 31;7:5. doi: 10.1186/1750-2187-7-5

Table 1.

Photobleaching time-constants and relative-FRET efficiencies in HEK-293 cells coexpressing SSTR2 and SSTR3

Treatment τavg(s) n E (%)
Control
 D - A
 18.3 ± 0.5
 55
 14.9 ± 1.2
D + A
 21.5 ± 0.7
 54
SST (1 nM)
 D - A
 18.6 ± 0.4
 58
 11.4 ± 1.4*
D + A
 21 ± 0.5
 56
SST (50 nM)
 D - A
 18.8 ± 0.4
 59
 12.1 ± 1.1
D + A
 21.4 ± 0.4
 60
SST (1 μM)
 D - A
 19.6 ± 0.6
 57
 10.9 ± 1.6*
D + A
 22 ± 0.2
 55
L-779976 (10 nM)
 D - A
 20.7 ± 0.3
 56
 10.4 ± 1.5*
D + A
 23.1 ± 0.3
 59
L-779976 (100 nM)
 D - A
 20.3 ± 0.4
 58
 12.1 ± 1.2
D + A
 23.1 ± 0.4
 54
L-796778 (25 nM)
 D – A
 19.5 ± 0.9
 59
 12.9 ± 1.7
D + A
 22.4 ± 0.7
 54
L-796778 (50 nM)
 D - A
 20.8 ± 0.5
 60
 8 ± 1.2*
D + A
 22.6 ± 0.6
 57
L-779976 (10 nM) + L-796778 (25 nM)
 D - A
 19.6 ± 0.5
 55
 11.3 ± 0.9*
D + A
 22.1 ± 0.2
 54
L-779976 (100 nM) + L-796778 (25 nM) D - A
 19.6 ± 0.5
 57
 12.9 ± 1.4
D + A 22.5 ± 0.5 54

D − A and D + A correspond to donor without and with acceptor, respectively; τavg refers to average of n photobleaching time constants; n is the total number of cells analyzed; E (%) is mean relative FRET efficiency. Mean ± SD is representative of three independent experiments (*, p < 0.05).