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. 2012 Jul 24;7(7):e41742. doi: 10.1371/journal.pone.0041742

Figure 2. An active form p53 can repress K14 promoter within the first 160 base pairs of the K14 promoter.

Figure 2

(A) H1299 cells were transfected with pcDNA 3.0, wild-type p53, mutant p53 248W, or ΔTAp53 expression vectors. Wild-type p53 up-regulated p21 expression, but p53 248 W and ΔTAp53 did not. β-actin expression was used as a loading control. Each well was loaded with 20 µg of total protein. (B) H1299 cells were co-transfected with the pK14-2000 promoter vector and pcDNA 3.0, wild-type p53, mutant p53 248 W, or ΔTAp53 expression vectors. The levels of luciferase promoter activity were normalized to that of pcDNA 3.0, which was set to 1. Wild-type p53 down-regulated pK14-2000 promoter activity, but p53 248 W and TAp53 did not. (C) H1299 cells were co-transfected with pK14-2000, pK14-269, pK14-187, or pK14-160 promoter vectors and pcDNA 3.0 or wild-type p53 expression vectors. The levels of luciferase promoter activity were normalized to that of pcDNA 3.0, which was set to 1. The activity of all K14 promoter truncations was down-regulated by p53.