Table 2.
Primer sets used for the 16S rRNA gene quantification ofA. muciniphila,F. prausnitzii,Enterobacteriaceae,Clostridiumcluster IV,BifidobacteriumandLactobacillusgroup by qPCR. Amplicon size, annealing and fluorescence acquisition temperature are also reported
| Target microorganism | Primer set | Sequence (5' to 3') | Product size (bp) | Annealing temp (°C) | Fluorescence acquisition temp (°C) | Reference |
|---|---|---|---|---|---|---|
|
Akkermansia muciniphila |
AM1 |
CAGCACGTGAAGGTGGGGAC |
349 |
63 |
88 |
[31] |
| |
AM2 |
CCTTGCGGTTGGCTTCAGAT |
|
|
|
|
|
Faecalibacterium prausnitzii |
Fprau223F |
GATGGCCTCGCGTCCGATTAG |
199 |
67 |
85 |
[32] |
| |
Fprau420R |
CCGAAGACCTTCTTCCTCC |
|
|
|
|
|
Enterobacteriaceae |
Eco1457F |
CATTGACGTTACCCGCAGAAGAAG |
195 |
63 |
87 |
[32] |
| |
Eco1652R |
CTCTACGAGACTCAAGCTTGC |
|
|
|
|
|
Clostridium Cl_IV |
S-*-Clos-0561-a-S-17 |
TTACTGGGTGTAAAGGG |
588 |
60 |
85 |
[33] |
| |
S-*-Clept-1129.a-A-17 |
TAGAGTGCTCTTGCGTA |
|
|
|
|
|
Bifidobacterium |
bif-164 |
GGGTGGTAATGCCGGATG |
523 |
60 |
90 |
[34] |
| |
bif-662 |
CCACCGTTACACCGGGAA |
|
|
|
|
|
Lactobacillus group |
Lac1 |
AGCAGTAGGGAATCTTCCA |
327 |
61 |
85 |
[35] |
| Lac2 | ATTYCACCGCTACACATG |