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. 2012 Jul 24;7(7):e40675. doi: 10.1371/journal.pone.0040675

Figure 3. FISH stains using same buffer component concentrations for EC and formamide buffers.

Figure 3

BCL2 in: A: 15% formamide buffer, B: 15% EC buffer and C: 45% formamide buffer with Cot-1 blocking, with different denaturation conditions on FFPE breast carcinoma tissue sections. Merge of green BCL2 DNA, red BCL2 DNA split probe signals and of blue DAPI staining. Denatured at 67°C for 10 minutes or 82°C for 5 minutes and hybridized at 45°C for 60 minutes. The images are taken with identical exposure times. Scale bar, 10 µm.