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. 2012 Jul 24;7(7):e41393. doi: 10.1371/journal.pone.0041393

Figure 1. Construction of the Δlgt strain.

Figure 1

(A) Schematic of the Sp_1410–1413 locus, with the TIGR4 genome gene number and the assigned gene names in parentheses when available. Arrows indicate transcriptional direction and lgt is shaded black. (B) Structure of the Sp_1410–1413 locus in the Δlgt mutant strain, showing replacement of lgt with an in-frame copy of cat which is shaded grey and position of primers used in (C). (C) Gel red stained agarose gels showing PCR analysis of two separately obtained Δlgt strains confirming replacement of lgt with cat. Primer pairs (Table 4) and the strain used for each reaction are given above each lane. (D) Gel red stained agarose gels of RT-PCR reactions using internal primers (Table 4) for each gene within the putative Sp_1410–1413 operon, confirming the non-polar deletion of lgt. Reactions not containing reverse transcriptase gave no products (not shown), and L marks the DNA ladder size marker with sizes listed on the left.