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. 2012 Jul 24;7(7):e41393. doi: 10.1371/journal.pone.0041393

Figure 3. Effect of the Δlgt mutation on surface accessability of S. pneumoniae lipoproteins.

Figure 3

(A) Flow cytometry analysis of the mean proportion of bacteria positive for IgG binding after incubation with immune sera containing high antibody titres towards lipoproteins of S. pneumoniae. Black columns represent wild-type strain and clear columns represent the Δlgt strain. Error bars represent SDs and P values were obtained using multiple ANOVA test with post-hoc analysis. (B) Immunofluorescence of the S. pneumoniae wild-type (WT), Δlgt and ΔPpmA strains using anti- PpmA antibody and FITC conjugated secondary antibody. (C) Immunofluorescence of the S. pneumoniae wild-type (WT), Δlgt and ΔPhtD strains using anti-PhtD antibody and Cy2 conjugated secondary antibody.