Figure 6. Growth of the wild-type and Δlgt strains in cation depleted media.

Assessed by measuring broth culture log₁₀ OD₅₈₀ over time. (A) to (C) Growth in cation depleted chelex-THY and (D) to (E) in CDM with and without cation supplementation: (A) and (D) with and without Mn²⁺ supplementation; (C) and (E) with and without Fe²⁺ supplementation; and (D) and (F) with and without combined Mn²⁺, Fe²⁺ and Zn²⁺ supplementation. Filled symbols represent growth of the wild-type strain, empty symbols growth of the Δlgt strain. Squares represent growth in unsupplemented media, inverted triangles in media supplemented with 50 µM Mn²⁺, diamonds in media supplemented with 50 µM Fe⁺² and circles in media supplemented with 50 µM Mn⁺², Fe⁺² and Zn⁺².