Figure 1. DMXAA does not induce any morphological changes in HUVEC cells whereas CA4 treated cells immediately contract.

(A) Images were collected from phase contrast time-lapse movies at indicated times. Original time-lapse imaging was taken every 30 sec for an hour in the presence of 500 µM of DMXAA, 100 nM of CA4 or without drug (control). Elapsed time indicated in hours:minutes. (B) Cell edges were drawn to measure the number of pixels within the cell edges. Individual cell areas were summed to measure total cell surface area at each time point. The total cell area for each time point was normalized by time point 0. At each condition, Cell surface areas were averaged from at least 3 different stage positions. Error bars were calculated as the standard deviation from the results of 3 independent experiments. (C) Images were collected from phase contrast time-lapse movies at 12 hours after drug treatment. Asynchronously grown cells were treated with 500 µM of DMXAA, 100 nM of CA4 or without drug (control). (D) From the time-lapse image, mitotic cells were counted after 12 hours of drug treatment. At least 200 cells from 3 different stage positions were counted at each condition. Error bars were calculated as the standard deviation from the results of 3 independent experiments.