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. 2012 Jul 24;7(7):e41023. doi: 10.1371/journal.pone.0041023

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Köpfer et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(I) Mutation sites N629Q (pore loop), F617Y, and Y616F (both: pore helix, P). (II) Inactivation properties of wild-type and mutant hERG channels. Inactivation time courses for the different hERG channels were recorded as shown. A conditioning pulse to +20 mV followed by a 100 ms hyperpolarizing pulse to −100 mV preceded various depolarizing pulses from −90 to +40 mV in 10 mV increments as illustrated by the pulse protocol on top. (III) (A) Exemplary wild-type (WT) hERG current traces elicited by 6 s depolarizing voltage steps from −100 to +40 mV followed by a hyperpolarizing pulse to −140 mV. Respective tail currents are shown enlarged at left. (B) Conductance-voltage relations determined from Boltzmann fits to normalized tail current amplitudes for hERG wild-type and Y616F and N629Q mutant channels. (C) Conductance-voltage relation for the mutant hERG channel F617Y. (D) and k parameters obtained under steady state conditions from the Boltzmann fits for wild-type and mutant channels are summarized at the bottom. *p<0.05 versus wild-type. (IV) (A) Deactivation time courses of wild-type and mutant hERG channels. Tail currents were elicited according to the pulse protocol shown on top. (B) Voltage dependence of mean deactivation time constants () (n = 4) for the different channels as indicated.

Inline graphic — (I) Mutation sites N629Q (pore loop), F617Y, and Y616F (both: pore helix, P). (II) Inactivation properties of wild-type and mutant hERG channels. Inactivation time courses for the different hERG channels were recorded as shown. A conditioning pulse to +20 mV followed by a 100 ms hyperpolarizing pulse to −100 mV preceded various depolarizing pulses from −90 to +40 mV in 10 mV increments as illustrated by the pulse protocol on top. (III) (A) Exemplary wild-type (WT) hERG current traces elicited by 6 s depolarizing voltage steps from −100 to +40 mV followed by a hyperpolarizing pulse to −140 mV. Respective tail currents are shown enlarged at left. (B) Conductance-voltage relations determined from Boltzmann fits to normalized tail current amplitudes for hERG wild-type and Y616F and N629Q mutant channels. (C) Conductance-voltage relation for the mutant hERG channel F617Y. (D) and k parameters obtained under steady state conditions from the Boltzmann fits for wild-type and mutant channels are summarized at the bottom. *p<0.05 versus wild-type. (IV) (A) Deactivation time courses of wild-type and mutant hERG channels. Tail currents were elicited according to the pulse protocol shown on top. (B) Voltage dependence of mean deactivation time constants () (n = 4) for the different channels as indicated.