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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Genes Brain Behav. 2012 Jun 15;11(6):727–739. doi: 10.1111/j.1601-183X.2012.00810.x

Figure 4. The rutabaga requirement for normal ethanol preference maps to the mushroom body neurons.

Figure 4

The indicated genotypes were examined in the CAFE assay for an ethanol preference. (a) In the presence of both the UAS-rut and the OK107 mushroom body Gal4 driver, the rut2080 ethanol preference phenotype is not significantly different than CS, and is significantly higher than the control rut2080 genotypes. (b) The MB-Gal80 transgene inhibits the expression of Gal4 in specifically mushroom body neurons (Krashes et al., 2007). In the presence of MB-Gal80, UAS-rut and OK107 did not display a significantly rescued ethanol presence in comparison to the rut2080; UAS-rut/+;; OK107/+ genotype, and also no significance in comparison to rut2080; MB-Gal80/+;; OK107/+ and rut2080; UAS-rut/MB-Gal80 genotype. (c) Similarly, the rut2080 ethanol preference phenotype is only rescued in the presence of both the UAS-rut and c772 mushroom body Gal4 driver. (d) The MB-Gal80 transgene similarly reduced the ethanol preference of rut2080 flies carrying the UAS-rut and c772 transgenes. However, even in the presence of MB-Gal80, UAS-rut and c772 Gal4 displayed a significantly increased ethanol preference, compared with the two negative control. (e) In the presence of both the UAS-rut and the 238y Gal4 driver, the rut2080 ethanol preference phenotype is significantly higher than the control rut2080 genotypes. In all genotypes, the ry506 allele was also present. (f) In the presence of MB-Gal80, UAS-rut and 238y-Gal4 display a significantly rescued ethanol presence in comparison to the rut2080; UAS-rut/+; 238y/+ genotype, and also no significance in comparison to rut2080; MB-Gal80/+;238y/+ and rut2080; UAS-rut/MB-Gal80 genotype. In all genotypes, a ry506 allele was taken in third chromosome. (g) In the presence of the UAS-rut and the combination of c305a + MB247 Gal4 drivers, the rut2080 ethanol preference phenotype is significantly higher than the control rut2080 genotypes. (h) The MB-Gal80 transgene similarly reduced the ethanol preference of rut2080 flies carrying the UAS-rut and c305a+MB247 transgenes, and also displayed no significance in comparison to rut2080; MB-Gal80/c305a; MB247/+ and rut2080; UAS-rut/MB-Gal80 genotype. Data are means ± S.E.M. “N.S.” means no significance. *P<0.05, **P<0.01 and ***P<0.001.