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. Author manuscript; available in PMC: 2013 Sep 25.
Published in final edited form as: Mol Cell Endocrinol. 2012 Apr 10;361(1-2):124–132. doi: 10.1016/j.mce.2012.03.024

Figure 8. The residual GR mRNA after knockdown of c-Myb is above the postulated threshold level required for GC mediated apoptosis.

Figure 8

697 sh-c-Myb cells were treated with 1 μg/ml Doxy for 48 h and 1 μM Dex/EtOH for 18 hr. 697 sh-GR cells were treated with 250 ng/ml Doxy for 48 h followed by 1 μM Dex/EtOH treatment for 18 hr. 697 cells and IM-9 cells were treated with 1 μM Dex/EtOH treatment for 18 hr. GR mRNA transcript measurements were performed via QRT-PCR with a probe and primer set specific for GR mRNA transcripts (containing the exon 5/6 junction). The data are normalized to endogenous 18S RNA levels. Error bars represent the standard error of the mean of three separate experiments. (*) denotes the cells that are resistant to GC-mediated apoptosis. The dotted line marks the apparent threshold level of GR transcripts that are required to trigger GC mediated apoptosis.