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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Gastroenterology. 2012 Apr 19;143(2):418–428. doi: 10.1053/j.gastro.2012.04.017

Figure 1. Distribution and phenotype of intestinal iNKT cells.

Figure 1

(A) Lymphocytes from the indicated sites were incubated either with αGalCer loaded or unloaded CD1d-tetramers, analyzed by flow cytometry and the frequencies of tetramer-positive cells within live TCRβ+CD44+CD8α−CD19− cells are shown. (B, C) Relative percentage of iNKT cells within total live lymphocytes (B) and their expression of CD4 and NK1.1 (C), from indicated sites. The graphs summarize data from 3–5 independent experiments, with 6–9 samples per group. (D) Representative expression of CCR9 on iNKT cells derived from the spleen (tinted, in both panels), IEL (dashed) or LPL (black line) from the small or large intestine. The numbers in histograms denote the geometric mean values for CCR9 on iNKT cells.