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Extended exposure of bAEC to TNFα or IL-6 inhibited β-actin expression at both mRNA and protein levels. Cultured bAEC were serum starved in the absence or presence of TNFα or IL-6 (20 ng/ml, each) for 24 h. Cells were then processed for real-time RT-PCR (A) or for Western blotting (B). *, P < 0.05 compared with EBM (incubated in basal medium only).
