Table 2.

Binding parameters for WT PROKR2 and ICL1 mutants

PROKR2	Bmax (cpm)	Log Kd (mol)
WT	11,911 ± 1014	−7.4 ± 0.03
R80C	4461 ± 463a	−7.3 ± 0.83
R85C	9898 ± 315	−7.6 ± 0.41
R85H	10,454 ± 2113	−7.4 ± 0.34

The amount of cell surface receptor is expressed as B_max and receptor affinity for the radioligand as K_d. Results are the mean for three independent experiments.

^a P < 0.05 compared with WT.