Fig. 3.

Antiangiogenic effect of ABT-510 and ABT-898 treatment in experimental prolactinomas. Pituitary vasculature was analyzed by CD31 immunostaining. A, Relative CD31-stained vascular area (CD31+ vascular area/total area) was higher in DES control and ABT-510-treated pituitaries compared with the pituitaries of rats in diestrus. *, P = 0.033; #, P = 0.037. B, Nonsignificant differences were found in the average size of CD31-stained vessels. C, Microvessel density (number of CD31 stained vessels per square millimeter) was increased in the DES control group compared with diestrus. *, P = 0.014. Treatment with ABT-510 and ABT-898 reduced microvessel density induced by DES; &, P = 0.01 and #, P = 0.027, respectively (n = 5, 7, 4, and 5).