Fig. 5.
Uterine developmental gene expression is altered in REAd/d mutant mice. A, mRNA levels of molecular markers (BMP2, Hoxa10, EGFR, and HGF) implicated in uterine development as monitored by qRT-PCR. B, The cell cycle inhibitor p21 mRNA expression levels are elevated in the uterus of REAd/d mice, as measured by qRT-PCR. C, p21 protein levels are elevated in the REAd/d uterus as observed by immunohistochemistry (IHC). D, Reduction of REA expression is correlated with increased p21 expression as measured by IHC (PND10 uterus). Scale bar, 200 μm. Values are mean ± sd (n = 10 per group), and mRNA levels are illustrated as relative expression normalized to 36B4 by wild-type PND5. *, P < 0.05; **, P < 0.01.