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. 2012 Jun 11;153(8):4030–4038. doi: 10.1210/en.2011-2113

Fig. 3.

Fig. 3.

A, Top, sequence of the 20-nucleotide region (−61 to −41) containing the CCAAT element (underlined bold) in human Dio2 promoter; bottom, sequence alignment of this region in various species; bold indicates C/EBP binding sites and TATA boxes. The numbers indicate the position of the two elements, relative to the most 5′ TSS in human Dio2. B, Luciferase assay of 1299 ATG Dio2 Luc promoter constructs in JEG3 cells. Cells were seeded in six-well plates and transfected with 1299 ATG WT Dio2 Luc, 1299 ATG CCAAT MUT Dio2 Luc, 1299 ATG TATA MUT Dio2 Luc, RSV β-gal, and C/EBPβ plasmids. At the end of the incubation, luciferase and β-galactosidase assays were performed. *, C/EBPβ vs. control, P < 0.001, Mann-Whitney test U test. Results represent the average value ± sd of three different experiments, each performed in triplicate. Bottom, Wild-type and mutant sequences (the underlined letters indicate the point mutations).