Fig. 4.

Hydrogen peroxide (H₂O₂) supports Msx2 induction in aortic adventitial myofibroblasts. A, The glutathione peroxidase-mimetic ebselen inhibits TNF induction of Msx2 (n = 4 per treatment group). Cultures were pretreated with ebselen for 30 min before treatment with TNF. B, Three hours of exogenous H₂O₂ treatment dose dependently increases Msx2 expression, phenocopying TNF (n = 3 per treatment group). C, Msx2 protein levels were also increased by H₂O₂ treatment (n = 4 per treatment group; P < 0.047; see also Supplemental Fig. 3).