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. 2012 Jul 15;26(14):1626–1642. doi: 10.1101/gad.191338.112

Figure 5.

Figure 5.

Ptbp2 represses aberrant alternative 3′ splice site selection. Ptbp2 silences alternative 3′ splice site utilization in Snap25 pre-mRNA. (A) Distribution of Ptbp2 CLIP tags in a region of Snap25 containing the alternative 5a and 5b exons. CLIP tag density is represented by a histogram where the black line indicates a CLIP tag density of 10 overlapping tags. (B) RT–PCR analysis of Snap25 5a/5b splicing in wild-type and Ptbp2-null brains. (C) Zoom-in of a region of Snap25 showing Ptbp2 CLIP tags and splice site utilization and intron retention identified from PCR products x, y, and z. (D) Zoom-in of C showing the position of the Ptbp2 CLIP cluster relative to the alternative 3′ splice site up-regulated in Ptbp2-null brains.