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Titers of IgG1 and IgG2a were measured separately by isotype-specific ELISA, using sera taken from mice which had received five different vaccination regimes at two time points (14 and 137 days after final vaccination). Vaccination regimes were as described in legend to Fig. 3 with doses as in legend to Fig. 1. Data are plotted as ratio of log titer of IgG2a/IgG1.
