Fig. 10.

Intracellular cytokine staining allowed detection of cells producing combinations of IFNγ, TNFα and IL-2. Vaccination regimes were as described in legend to Fig. 3 with doses as in legend to Fig. 1. Gating strategy is illustrated in supplementary Figure 1. Panel A: Proportions of CD8⁺ T cells positive for at least one cytokine (usually IFNγ) or positive for a second cytokine (usually TNFα) 2 weeks after final vaccination. Produced using SPICE software (M. Roederer, NIH, USA). Panel B: Proportions of all CD8⁺ T cells which were positive for two cytokines.