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. Author manuscript; available in PMC: 2012 Jul 25.
Published in final edited form as: Nature. 2011 Jul 6;475(7354):106–109. doi: 10.1038/nature10189

Figure 3. Activation of Nrf2 by K-RasG12D occurs via the Raf-MEK-ERK-Jun pathway.

Figure 3

a, ROS levels following treatment of K-RasG12D/+ MEFs with AZD6244. LSL-K-RasG12D MEFs were treated with DMSO or 0.1uM AZD6244, infected with Ad-mock (K-RasLSL/+) or Ad-cre (K-RasG12D/+) and assayed after 72 hours. b, Analysis of antioxidant gene expression following treatment of K-RasLSL/+ and K-RasG12D/+ MEFs with AZD6244 for 24 hours. c, Control of Nrf2 transcription by AP-1 family members. K-RasLSL/+ and K-RasG12D/+ MEFs were transfected with siRNA and assayed for Nrf2 expression after 48 hours. d, Western blot of Jun and actin protein levels in LSL-K-RasG12D and LSL-B-RafV619E MEFs. K-RasLSL/+ and K-RasG12D/+ MEFs were treated with DMSO or 0.1uM AZD6244 for 24 hours. e, ROS levels following Jun depletion with siRNA. LSL-K-RasG12D MEFs were transfected with non-targeting (NT) or Jun siRNA, infected with Ad-mock or Ad-cre and assayed after 48 hours for DCF oxidation. Data are representative of 3 independent experiments.