Figure 4.

Native RNA structures do not protect RNA from H₂O₂-mediated oxidation in vitro.

(A) tRNA was isolated from total RNA by isopropanol differential precipitation (see Figure 3). Native and denatured tRNA samples were incubated with indicated concentrations of H₂O₂ in vitro and 8-oxo-G levels were determined as described in the Materials and methods section. (B) rRNA was prepared from ribosomes as shown in Figure 3. Native and denatured rRNAs were treated with indicated concentrations of H₂O₂ in vitro and 8-oxo-G level was determined. The mean and standard error of at least three replicates were plotted.