Figure 2. Comparison of BeAr436087 and FL93-939 replication and dissemination.
6-week-old CD1 mice were infected with 103 pfu of BeAr436087 (open circle) or FL93-939 (closed circle) in each hind footpad. Mice were perfused with PBS-1% DCS prior to tissue harvesting, homogenization and mechanical disruption. Virus titers in serum and tissue homogenates were determined by BHK-21 plaque titration: A) popliteal draining lymph node; B) serum; C) spleen; D) knee joint (femur epiphyses and metaphyses); E) bone aspirate (femur); F) brain. LD = limit of detection of the plaque assay and error bars represent standard deviations.
