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. Author manuscript; available in PMC: 2012 Jul 25.
Published in final edited form as: Kidney Int. 2010 Oct 6;79(3):288–299. doi: 10.1038/ki.2010.381

Figure 4. Immunofluorescence shows colocalization of Toll-like receptor 4 (TLR4) and von Willebrand’s factor on the vasa rectae of the ischemic inner stripe of the outer medulla (ISOM).

Figure 4

The ischemic kidney was studied at 4 h reperfusion. The same tissue section is shown in all panels, but different input and output light filters were used. Blue is 4,6-diamidino-2-phenylindole. (a) TLR4 visualized with phycoerythrin-conjugated monoclonal rat anti-mouse TLR4 (Santa Cruz, sc 13591). (b) Endothelia visualized with anti-von Willebrand’s antibody and then goat anti-rabbit IgG-Cy3. (c) Overlap of a and b.