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. Author manuscript; available in PMC: 2012 Jul 25.
Published in final edited form as: Kidney Int. 2010 Oct 6;79(3):288–299. doi: 10.1038/ki.2010.381

Figure 6. Endothelial Toll-like receptor 4 (TLR4) is directly increased by stress in vitro.

Figure 6

Confluent MS1 murine endothelial cells were treated with H2O2 (a) or tunicamycin in Earl’s Balanced Solution (EBSS) (b) for 30 min, and assayed for TLR4 mRNA immediately (zero time) or recultured in complete medium without H2O2 or tunicamycin and assayed after 4 or 24 h. The y axis is the TLR4/GAPDH mRNA determined by quantitative reverse transcription-PCR and analyzed by the comparative Ct method (see Materials and Methods section). The calibrator gene is TLR4 expression in the untreated control in EBSS for 30 min and in complete medium at the given recovery time. Means and standard errors are shown; *P<0.05.