Fig. 1.

Slo interacts with receptor for activated PKC (Rack1) in yeast 2-hybrid experiments. The schematic figure shows the COOH-terminal region of Slo used for the yeast 2-hybrid experiments. The region extends from amino acid 746-1114 and includes the RCK domains 1 and 2 immediately after the transmembrane domain. Also shown is a schematic of chick Rack1 including the amino acids 88-317 identified in the yeast 2-hybrid experiment. The interaction of these regions occurred under stringent conditions (-His/-Ade/+α-galactosidase).