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. 2012 Apr 25;303(2):C143–C150. doi: 10.1152/ajpcell.00062.2012

Fig. 7.

Fig. 7.

Increased Slo surface expression in response to PKC activation is not due to direct phosphorylation of Slo. The following phospho-null mutants S642A, S1065A, S1076A, and SS1076/77AA were created and tested for Slo surface expression with or without the PMA treatment. All but 1 (S642A) of these mutants showed a change in baseline surface expression, and all of them responded to PMA treatment with an increase in Slo surface expression. In addition the following phospho-mimetic mutants S1065D, S1076/77DD, and S1076D were tested. They also didn′t alter Slo surface expression and responded to PMA treatment with increased Slo expressed on the surface of the cell. These results were replicated in 3 independent experiments.