Fig. 1.

Proinflammatory cytokine-induced NF-κB reporter activity. A: C2C12 cells were transfected with a NF-κB reporter, differentiated for 3 days, and treated with each cytokine (10 ng/ml) for the times indicated up to 8 h. Fold changes are compared with activity in the absence of cytokine treatment (statistically different from 2- to 8-h treatment, except IL-6). B: L6 myoblasts differentiated for 3 days and treated the same as in A. C: 3-day differentiated C2C12 myotubes were treated with increasing concentration of IL-6 for 4 h. TWEAK, TNF-related weak inducer of apoptosis; RLU, relative light units. Values are means ± SE from 3 separate wells of myotubes. *P < 0.05 vs. control.