Fig. 3.

TNF-α-induced myotubes atrophy is reversed by p65 inhibition. C2C12 myotubes were treated for 48-h TNF-α, with or without HLN (1 μM), compared with control. A: immunocytochemistry of myotubes after 48 h of treatment stained by anti-myosin. B: myotube diameter measured at 0, 24, and 48 h of TNF-α treatment, with or without HLN, compared with no cytokine control group. C: HLN treatment alone had no effect on myotube diameter. Values are means ± SE. *P < 0.05 vs. control. †P < 0.05 vs. cytokine.