Fig. 6.

Blocking of autocrine IL-6 failed to inhibit TNF-α or IL-1β-induced atrophy. Three-day differentiated C2C12 myotubes were treated with cytokines in the absence or presence of IL-6 blocking antibody. Diameter was measured for myotubes treated with IL-6 (A), TNF-α (B), or IL-1β (C), with or without IL-6 blocking antibody and control myotubes. D: IL-6 protein concentration in cell culture medium (at 24 h of treatment) from control, TNF-α, or IL-1β-treated myotubes. Values are means ± SE. *P < 0.05 vs. control. †P < 0.05 vs. IL-6.