Fig. 1.

The distribution of histone deacetylase 4 (HDAC4) in growth plate. Immunofluorescent histochemistry was performed with 5-μm-thick sections of the proximal tibia growth plate (from 1 day postpartum mice) using anti-HDAC4 antibody. A: in the proliferation zone, HDAC4 is located in the nucleus but it translocates gradually to the cytoplasm in the maturation/prehypertrophic zone. Normal goat IgG was used in place of HDAC4 primary antibody as negative control. No positive staining was detected for HDAC4 in the negative controls. Scale bar, 100 μm. B: the boxed region in Ac (top right) is represented at a higher magnification (×40). Scale bar, 20 μm. Blue, nucleus; red, HDAC4; purple, overlap of blue and red. C: the boxed region in Ab (top middle) is represented at a higher magnification (×40). Scale bar, 20 μm. Red, HDAC4.