Fig. 2.

Excitation-transcription coupling of CPI-17 mRNA in AoSMC. Extent of CPI-17 mRNA in AoSMC was determined by qRT-PCR using β-tubulin as reference. A: quiescent cells were stimulated overnight with TGFβ (5 ng/ml), IL-1β (20 ng/ml), IL-6 (10 ng/ml), TNFα (10 ng/ml), and sorbitol (0.2M) (n = 8–9) and subjected to qRT-PCR assay. B: unstimulated and sorbitol stimulated cells were treated overnight with SP600125 (SP; 10 μM) and SB203580 (SB; 10 μM) (n = 6). C: cells in the growth medium supplemented with 10% FBS were harvested with calphostin-C (Cal; 50 nM), GF109203x (GFx; 3 μM), Go6976 (Go; 1 μM), H1152 (H; 3 μM), and Y27632 (Y; 10 μM; n = 9). **P < 0.05 and #P < 0.05, compared with untreated and stimulated cell, respectively.