Skip to main content
. 2012 Apr 25;303(1):C58–C68. doi: 10.1152/ajpcell.00118.2012

Fig. 3.

Fig. 3.

Luciferase reporter gene assay for CPI-17 gene 5′-flanking region. pGL4.20 luciferase reporter gene vectors used in the assay are illustrated at left. pGL4.20 vector and CMV-secreted embryonic alkaline phosphatase (SEAP) were cotransfected for 72 h under the growth condition and subjected to luciferase and SEAP assay. Promoter activity was defined as luciferase activity/SEAP activity and normalized against mouse 510 bp (A and B) and human 1 kb (C). WT, wild type. *P < 0.05 against mouse 510 bp or human 1 kb (n = 6–12).