Table 1.
List of primers/probes for qRT-PCR and qChIP
Target | Sequence |
---|---|
qRT-PCR | |
CPI-17 CDS* | ACATGCCAGATGAGGTCAACATCG |
AAGTCCTCTGTGGGATTCAGGCAA | |
Tub-ß CDS | AGATGGCAGTCACCTTCATCGGAA |
TGTTGCTCTCAGCTTCGGTGAACT | |
Myocardin CDS | GTCGAGTCCAACAGTTCCGGA |
CTCACTGTCGGTGGCATAGTG | |
KLF4 CDS | CTTTCCTGCCAGACCAGATG |
GGTTTCTCGCCTGTGTGAGT | |
sm22α CDS | AGGCAGCTGAGGATTATGGA |
CTGCCCAAAGCCATTACAGT | |
sm-α-actin CDS | CATCAGGAAGGACCTCTATGC |
CTGATCCACATCTGCTGGAAG | |
Rel-A CDS | CCACTAAGACGCACCCCACC |
GCTTGCTCCAGGTCTCGCTT | |
ChIP | |
CPI-17 proximal | TGCACGTCTCTGTAGGAAGG |
CTTGCGCTTGGGTCTCTAAC | |
FAM-CGGGTCCCAGAACACGAGCC-IABFQ | |
CPI-17 distal | GCCAGAGGGCGTAACAGG |
GTCCTGCGGGGATACAGAT | |
5HEX-CGGAGCCCTCTGAGATGGGC-IABFQ |
ChIP, chromatin immunoprecipitation; q, quantitative; KLF4, Kruppel-like factor 4; sm, smooth muscle.
CDS is coding sequence. Primers and Tacman probes were designed using PrimerQuest software at www.IDTDNA.com.