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. 2012 May 2;303(1):C102–C111. doi: 10.1152/ajpcell.00009.2012

Fig. 6.

Fig. 6.

Effect of inhibition of SG formation on apoptotic sensitivity in polyamine-deficient IECs. A: images of apoptotic cell death after treatment with and without TNFα/CHX for 4 h. Cells were treated with DFMO for 4 days and then transfected with C-siRNA, siSort1, or siTIA-1 for 48 h in the presence of DFMO. After cells were treated with arsenite for 45 min, they were exposed to TNFα/CHX for 4 h: control cells transfected with C-siRNA (a), DFMO-treated cells transfected with C-siRNA and then exposed to arsenite (b), DFMO-treated cells transfected with siSort1 and treated with arsenite (c), DFMO-treated cells transfected with siTIA-1 and treated with arsenite (d), and DFMO-treated cells cotransfected with siSort1 and TIA-1 and treated with arsenite (e). Original magnification, ×150. B: percentage of apoptotic cells as described in A. Values are means ± SE of data from 6 samples. *,+P < 0.05 compared with No-TNFα/CHX and DFMO-treated cells transfected with C-siRNA and then treated with TNFα/CHX, respectively.