Table 1.

Description of batch and chemostat cultures of “Ca. Methylacidiphilum fumariolicum” SolV.

Culture	Nitrogen source	Growth rate (h−1)	Doubling time (h)	Yield (g DW/mol CH4)	Limitation	O2 concentrationb (%)
Batch	Ammoniuma	μmax = 0.07	10	6.5	No	18
	Nitrate	μmax = 0.04	17	n.d.	No	18
	N2	μmax = 0.025	27	n.d.	Nitrogen	<1
Chemostat	N2a	μ = 0.017	40	3.5	Methane	0.5
	Ammoniuma	μ = 0.017	40	4.9c	Oxygen	<0.03

^aCells used for transcriptome analysis. In all other Tables referred to as “Cells at μ_max,” “N₂ fixation,” and “O₂ limitation.”

^bFor batch cultures initial headspace oxygen concentrations are given, while for the chemostat cultures measured dissolved oxygen concentration (dO₂) is expressed as % oxygen saturation (100% equals 800 μmol/l at 55°C).

^cCalculated from OD₆₀₀ comparisons at steady state.