Table 4.
Transcription of genes involved in nitrogen fixation in “Ca. M. fumariolicum” strain SolV.
Enzyme | Gene name | GenBank identifier | Expression level (RPKM) |
||
---|---|---|---|---|---|
Cells at μmax | N2 fixing cells | O2 limited cells | |||
Nitrogenase iron protein | nifH | Mfum_690040 | 69 | 22651 | 80 |
Nitrogenase molybdenum-iron protein alpha chain | nifD | Mfum_690039 | 87 | 14359 | 65 |
Nitrogenase molybdenum-iron protein beta chain | nifK | Mfum_690038 | 35 | 3348 | 34 |
Nitrogenase Mo/Fe cofactor biosynthesis protein NifE | nifE | Mfum_690037 | 77 | 5370 | 67 |
Nitrogenase Mo/Fe cofactor biosynthesis protein NifN | nifN | Mfum_690036 | 64 | 3677 | 52 |
Protein NifX | nifX | Mfum_690035 | 100 | 2831 | 26 |
Nif-specific regulatory protein | nifA | Mfum_690018 | 123 | 1080 | 109 |
Nitrogenase Mo/Fe cofactor biosynthesis protein NifB | nifB | Mfum_690029 | 19 | 3316 | 14 |
Pyruvate-flavodoxin oxidoreductase | nifJ | Mfum_940083 | 149 | 133 | 185 |
NifQ family protein | nifQ | Mfum_690020 | 65 | 270 | 22 |
Cysteine desulfurase | nifS1 | Mfum_690022 | 93 | 801 | 87 |
nifS2 | Mfum_90010 | 159 | 133 | 116 | |
nifS3 | Mfum_190023 | 681 | 491 | 486 | |
nifS4 | Mfum_970062 | 162 | 275 | 145 | |
nifS5 | Mfum_310028 | 226 | 151 | 264 | |
NifU-like protein involved in FeS cluster formation | nifU | Mfum_310029 | 54 | 16 | 79 |
Nitrogenase-stabilizing/protective protein NifW | nifW | Mfum_690011 | 62 | 2260 | 43 |
NifZ domain protein | nifZ | Mfum_690023 | 158 | 1373 | 50 |
Electron transfer flavoprotein beta chain | fixA | Mfum_690010 | 60 | 3311 | 73 |
Electron transfer flavoprotein alpha chain | fixB | Mfum_690009 | 81 | 3043 | 69 |
Flavoprotein-ubiquinone oxidoreductase | fixC | Mfum_690008 | 101 | 3632 | 100 |
Ferredoxin-like protein | fixX | Mfum_690007 | 126 | 2917 | 135 |
Nitrogen fixation protein FixU | fixU | Mfum_690015 | 164 | 2265 | 91 |
FeS cluster assembly scaffold protein, HesB/SufA family | sufA1 | Mfum_690026 | 40 | 2301 | 22 |
sufA2 | Mfum_810040 | 149 | 43 | 71 | |
sufA3 | Mfum_1020116 | 214 | 143 | 183 | |
FeS cluster assembly protein SufB | sufB | Mfum_970056 | 1099 | 2409 | 622 |
FeS cluster assembly protein SufD | sufD | Mfum_970057 | 663 | 2169 | 279 |
FeS cluster assembly protein SufE family | sufE | Mfum_110022 | 375 | 209 | 219 |
FeS4 cluster protein with leucine rich repeats | Mfum_690024 | 88 | 1884 | 61 | |
Ferredoxin-like protein in nif region | frxA | Mfum_690027 | 112 | 7892 | 46 |
Uptake hydrogenase large subunit | hupB | Mfum_50004 | 92 | 372 | 1840 |
Uptake hydrogenase small subunit | hupS | Mfum_50003 | 186 | 997 | 2955 |
Nickel/iron-hydrogenase I, small subunit | hyaA | Mfum_870019 | 2380 | 4157 | 4280 |
Nickel/iron-hydrogenase I, large subunit | hyaB | Mfum_870018 | 2566 | 1879 | 3595 |
Ni,Fe-hydrogenase I cytochrome b subunit | hyaC | Mfum_50005 | 122 | 593 | 984 |
[NiFe] hydrogenase Ni incorporation protein HypA | hypA | Mfum_730023 | 119 | 110 | 95 |
[NiFe] hydrogenase Ni incorporation-associated protein HypB | hypB | Mfum_870009 | 421 | 335 | 692 |
[NiFe] hydrogenase metallocenter assembly protein HypC | hypC | Mfum_870006 | 830 | 600 | 321 |
[NiFe] hydrogenase expression/formation protein HypD | hypD | Mfum_870005 | 1159 | 893 | 1246 |
[NiFe] hydrogenase metallocenter assembly protein HypE | hypE | Mfum_870004 | 424 | 476 | 759 |
[NiFe] hydrogenase metallocenter assembly protein HypF | hypF | Mfum_870007 | 92 | 81 | 167 |
Hydrogenase expression protein HupH | hupH | Mfum_50006 | 160 | 631 | 879 |
Hydrogenase expression/formation protein HoxQ | hoxQ | Mfum_730017 | 177 | 169 | 136 |
Hydrogenase maturation protease | hycI | Mfum_730022 | 69 | 44 | 89 |
The mRNA expression is expressed as RPKM according to Mortazavi et al. (2008). Changes in expression in the chemostat cultures (N2 fixing cells or O2 limited cells) compared to batch culture cells growing at μmax are indicated by shading: up regulation >2 times (dark gray), down regulation <0.5 (light gray).