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. 2012 Jul 25;2:77. doi: 10.3389/fonc.2012.00077

Figure 4.

Figure 4

DFNA5 cytotoxicity is enhanced in cells lacking mitochondrial functions. (A) Growth profiles of fis1Δ cells expressing C-terminally HA-tagged wtDFNA5 (dark gray squares) or mutDFNA5 (black triangles) or transformed with an empty plasmid (light gray circles). (B,C) Quantification of ROS accumulation using DHE staining (panel B) or AV/PI positive cells (panel C) in mid-exponential fis1Δ cultures without (contr.) or with expression of HA-tagged wtDFNA5 or mutDFNA5. (D) Fluorescence microscopy pictures of fis1Δ cells expressing EGFP or displaying inclusions of wtDFNA5-EGFP or mutDFNA5-EGFP and co-transformed with Mito-RFP to visualize mitochondria. (E) Growth profiles of por1Δ cells expressing HA-tagged wtDFNA5 (dark gray squares) or mutDFNA5 (black triangles) or transformed with an empty plasmid (light gray circles). Significances were assayed using unpaired t-tests. For the AV/PI co-staining of the fis1Δ cultures the following significant p-values were obtained when making the comparison with the control and mutDFNA5: AV+/PI+ **. (* = p < 0.05; ** = p < 0.001).