MMPs mRNA expression. Uterine tissue homogenate from virgin, mid-Preg, and late-Preg rats were prepared for real-time RT-PCR. The mRNA expression of MMP-2 (A), MMP-9 (B), and MMP-7 (C) was measured and normalized to the housekeeping gene for actin. Data represent means ± SE, n = 4 to 6. *Significantly greater (P < 0.05) than corresponding measurement in virgin rats. §Significantly less (P < 0.05) than corresponding measurements in virgin rats.