Fig. 5.

CaMKIV-cAMP response element binding protein (CREB) signaling pathway mediates autophagy. A: Western blot analysis of p-CREB in WT and CaMKIV KO mice after I/R. Values are means ± SE. *P < 0.05, WT vs. KO at the same time point. B: p-CREB in hypoxic hepatocytes transfected with CaMKIV siRNA. C: hepatocytes transfected with control vector, or CaMKIV-dCT. Images were representative of three experiments with similar results. D: Western blot for LC3B in hypoxia-stimulated hepatocytes after transfection with CaMKIV-dCT. When exposed to 1% hypoxia, cells were concomitantly treated with or without a CREB inhibitor (2.5 μM) for 1 h.