Fig. 2.

SK-CO15 cells have higher transfection efficiency than Caco-2 cells. A: SK-CO15 and Caco-2 cells transiently transfected with peGFP (green fluorescence protein) are shown. Top and bottom: brightfield and fluorescence images, respectively. Representative images are shown from 3 independent transfection. B: expression levels of hemagglutinin-tagged lysophosphatidic acid receptor type 5 (HA-LPA₅) in SK-CO15 and Caco-2 cells were compared by Western blot analysis. The expression levels of HA-LPA₅ relative to β-actin are shown at bottom. LPA-mediated activation of Erk1/2 was determined by Western blot analysis, and the relative levels of Erk1/2 activation are indicated. pErk1/2, phosphorylated Erk1/2. Representative images are shown from 3 independent experiments.