Fig. 6.

Regulation of NHE3 in SK-CO15 cells. SK-CO15 cells were treated with 1 μM dexamethasone (Dex) for 24 or 48 h, and the expression levels of NHE3 mRNA (A) and protein (B) were determined by qRT-PCR and Western blot analysis, respectively (n = 3; *P < 0.05). C: NHE3 activity in SK-CO15 cells treated with 1 μM Dex was determined. Results are presented as means ± SE (n = 9, *P < 0.05 compared with control). D: SK-CO15 cells transfected with pCDH or pCDH-LPA₅ were treated with 1 μM LPA for 5 min and NHE3 activity was determined as described earlier (n = 9; *P < 0.05 compared with control). E: cells were treated with 10 μM forskolin (FSK) for 30 min prior to NHE3 activity measurement (n = 9; *P < 0.05).